Invitrogen™ ReadyProbes™ 2.5% Normal Goat Serum (1X)

Catalog No.	Quantity
R37624	50 mL

Catalog No. R37624 Supplier Invitrogen™ Supplier No. R37624

Description

Ready-to-use non-specific blocker for use in immunohistochemistry (IHC), immunocytochemistry (ICC), and immunofluorescence (IF) experiments

• ReadyProbes 2.5% Normal Goat Serum is a ready-to-use non-specific blocker for use in immunohistochemistry (IHC), immunocytochemistry (ICC), and immunofluorescence (IF) experiments, usually when a goat secondary antibody is used.
• Incubating cells/tissue with diluted serum from the same species as the host of the secondary antibody typically reduces non-specific binding.
• Simply cover the tissue section or cells with a few drops of ReadyProbes 2.5% Normal Goat Serum and incubate for 30–60 minutes at room temperature.
• Ready Probes 2.5% Normal Goat Serum can also be used to dilute primary and secondary antibodies.
• Ready Probes 2.5% Normal Goat Serum is provided as a ready-to-use reagent with no further dilution or preparation required.
• The serum is collected from healthy adult animals and the heat-treated, centrifuged, and filtered. It is then checked for cross reactivity using an appropriate immunoassay.
• The serum is diluted to 2.5% (v/v) in buffer, with 0.08% sodium azide added as a preservative.

Specifications

• Content And Storage: Provided as 50-mL solution at ready-to-use (1X) concentration in a clear plastic dropper bottle.
  
  Store at 2–8°C.
  
  When stored as directed, product can be used for 6 months after receipt.
  
  
• Species: Goat
• Form: Liquid
• Product Type: Goat Serum
• Quantity: 50 mL
• Product Line: ReadyProbes
• Reagent Type: Blocking Reagent
• Shipping Condition: Room Temperature or Wet Ice

Frequently Asked Questions (FAQs)

Can I use ReadyProbes 2.5% Normal Goat Serum (1X) as an antibody diluent for both primary and secondary antibodies?

Yes, ReadyProbes 2.5% Normal Goat Serum (1X) can be used as an antibody diluent for both primary and secondary antibodies, except for HRP-conjugated antibodies. The sodium azide added as a preservative is an inhibitor of horseradish peroxidase.

How many drops of ReadyProbes 2.5% Normal Goat Serum (1X) should I add to a sample?

Add a sufficient number of drops of ReadyProbes 2.5% Normal Goat Serum (1X) to completely cover the sample.

When would I use goat, horse, or chicken serum for blocking versus FBS or BSA?

For blocking, you should use a serum of the same or closely related species of the secondary antibody being used. If using a secondary antibody that was raised in goat or sheep, use goat serum (Cat. No. R37624 or 31873) for blocking; if using a secondary antibody raised in donkey or horse, use horse serum (Cat. No. R37625 or 31874) for blocking; if using a secondary antibody raised in chicken, use chicken serum (Cat. No. R37626) for blocking. FBS and BSA are alternatives when a specific species of serum is not available.

For Research Use Only. Not for use in diagnostic procedures.