R&D Systems™ Recombinant Cynomolgus Monkey TIM-3 Fc Chimera Protein

Catalog No.
7914TM050	50ug; Carrier Free

Catalog No. 7914TM050 Supplier R&D Systems™ Supplier No. 7914TM050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-3 μg/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human Galectin-9 (Catalog # 2045-GA ) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey TIM-3 Chimera that produces 50% of the optimal binding response is 0.2-1.2 μg/mL.

Specifications

• Accession Number: EHH54703
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 84868
• Molecular Weight (g/mol): M.W. (Observed): 60-75 kDa, reducing conditions; M.W. (theoretical): 46.3 kDa (monomer)
• Name: TIM-3 Fc Chimera
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived cynomolgus monkey TIM-3 protein Cynomolgus Monkey TIM-3 (Ser22-Arg201) (N-terminus) Accession # EHH54703 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CD366, FLJ14428, HAVcr-2, HAVCR2, hepatitis A virus cellular receptor 2, kidney injury molecule-3, KIM-3, SPTCL, T cell immunoglobulin mucin 3, T cell immunoglobulin mucin-3, T-cell immunoglobulin and mucin domain-containing protein 3, TIM 3, Tim-3, TIM3, TIM3 T-cell membrane protein 3, TIMD3, TIMD-3, TIMD3KIM-3
• Gene Symbol: HAVCR2
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: TIM-3