R&D Systems™ Recombinant Cynomolgus PD-1 Fc Chimera Protein

Catalog No.	Quantity
85-78P-D050	50 μg

Catalog No. 85-78P-D050 Supplier R&D Systems™ Supplier No. 8578PD050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its binding ability in a functional ELISA. When Recombinant Human PD-L2/B7-DC Fc Chimera (Catalog # 1224-PL ) is coated at 0.25 μg/mL (100 μL/well), the concentration of Recombinant Cynomolgus Monkey PD-1 Fc Chimera that produces 50% optimal binding response is 0.03-0.15 μg/mL.

Specifications

• Accession Number: NP_001271065
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 5133
• Molecular Weight (g/mol): M.W. (Observed): 57-68 kDa, reducing conditions; M.W. (theoretical): 43 kDa (monomer)
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived cynomolgus monkey PD-1 protein Cynomolgus Monkey PD-1 (Leu25-Gln167) (N-terminus) Accession # NP_001271065 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: CD279, CD279 antigen, hPD-1, PD1, PD1hPD-l, PDCD1, programmed cell death 1, programmed cell death protein 1, Protein PD-1, SLEB2
• Common Name: PD-1
• Gene Symbol: PDCD1
• Biological Activity: Measured by its binding ability in a functional ELISA. When Recombinant Human PD-L2/B7-DC Fc Chimera is coated at 0.25μg/mL (100μL/well), the concentration of Recombinant Cynomolgus Monkey PD-1 Fc Chimera that produces 50% optimal binding response is typically 0.03-0.15μg/mL.
• Conjugate: Unconjugated
• Species: Cynomolgus Monkey
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Expression System: Human embryonic kidney cell, HEK293-derived Cynomolgus (Leu25-Gln167) Accession # NP_001271065, N-terminus; IEGRMD Human IgG1 (Pro100-Lys330), C-terminus
• Purity or Quality Grade: >95%, by SDS-PAGE with silver staining.
• Protein: PD-1