R&D Systems™ Recombinant F. meningosepticum PNGase F Protein

Description

Effective removal of N-linked oligosaccharides from glycoproteins. Glycerol-free, suitable for downstream MS and HPLC.

Measured by its ability to deglycosylate ribonuclease B under denatured conditions. >50% ribonuclease B (10 μg) is deglycosylated by 2.5 ng rFmPNGase F within 30 minutes, as measured under the described conditions.

Specifications

• Accession Number: P21163
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Molecular Weight (g/mol): M.W. (Observed): 34 kDa, reducing conditions; M.W. (theoretical): 36 kDa
• Quantity: 20 μg
• Source: E. coli-derived f. meningosepticum PNGase F protein Ala41-Asn354 with N-terminal Met and 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Common Name: PNGase F
• Biological Activity: Measured by its ability to deglycosylate ribonuclease B under denatured conditions. >50% ribonuclease B (10μg) is deglycosylated by 2.5 ng rFmPNGase F within 30 minutes, as measured under the described conditions.
• Conjugate: Unconjugated
• Species: F. meningosepticum
• Recombinant: Recombinant
• Expression System: E. coli-derived Ala41-Asn354 with N-terminal Met and 6-His tag
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: PNGase F