R&D Systems™ Recombinant Human 4-1BB/TNFRSF9 Fc Chimera Protein

Description

Extensive quality control produces lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Binding Activity

Measured by its binding ability in a functional ELISA. When recombinant human 4-1BB Fc Chimera is Immobilized at 10 ng/mL (100 μL/well), the concentration of recombinant human 4-1BB Ligand (Catalog # 2295-4L) that produces 50% optimal binding response is found to be approximately 0.5-2.5 ng/mL.

Specifications

• Accession Number: Q07011
• For Use With (Application): Binding Activity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 3604
• Molecular Weight (g/mol): M.W. (Observed): 50-65 kDa, reducing conditions; M.W. (theoretical): 44.8 kDa (monomer)
• Name: 4-1BB/TNFRSF9/CD137 Fc Chimera
• Quantity: 100 μg
• Source: Mouse myeloma cell line, NS0-derived human 4-1 BB/TNFRSF9/CD137 protein Human 4-1 BB (Leu24-Gln186) (N-terminus) Accession # Q07011 DIEGRMD Human IgG1 (Pro100-Lys330) 6-His tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: TNFRSF9
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: 4-1 BB/TNFRSF9/CD137