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R&D Systems™ Recombinant Human 4-1BB/TNFRSF9 His-tag Protein

Catalog No. 92204B100
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92-204-B100 Quantity: 100μg
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its binding ability in a functional ELISA. When Recombinant Human 4-1BB/TNFRSF9/CD137 is immobilized at 50 ng/mL, 100 μL/well, the concentration of Recombinant Human 4-1BB Ligand/TNFSF9 (Catalog # 2295-4L ) that produces 50% of the optimal binding response is approximately 2.5-12.5 ng/mL.

Order Info

The product is shipped at ambient temperature.

Specifications

Accession Number Q07011
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Gene ID (Entrez) 3604
Molecular Weight (g/mol) M.W. (Observed): 25-38 kDa, reducing conditions; M.W. (theoretical): 18 kDa
Name 41BB, 4-1BB, 4-1BB ligand receptor, CD137, CD137 antigen, CD137MGC2172, CDw137, FLJ43501, ILA, ILAhomolog of mouse 4-1BB, induced by lymphocyte activation (ILA), interleukin-activated receptor, homolog of mouse Ly63, receptor protein 4-1BB, T cell antigen
Quantity 100 μg
Source Chinese Hamster Ovary cell line, CHO-derived human 4-1 BB/TNFRSF9/CD137 protein Leu24-His183, with a C-terminal 6-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Regulatory Status RUO
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Biological Activity 2.5 to 12.5ng/mL (ED 50)
Product Type Recombinant protein
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Protein Tag No
Reconstitution Reconstitute at 500 μg/mL in PBS.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein 4-1 BB/TNFRSF9/CD137
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