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R&D Systems™ Recombinant Human ACE-2 Fc Chimera Biotinylated Protein

Catalog No. BT10544020
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20 μg
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BT10544020 20 μg
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Catalog No. BT10544020 Supplier R&D Systems™ Supplier No. BT10544020
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Measured by its binding ability in a functional ELISA with Recombinant SARS-CoV-2 Spike RBD Fc Chimera (Catalog # 10499-CV ). Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007 ). The specific activity is >450 pmol/min/μg, as measured under the described conditions.

Specifications

For Use With (Application) Bioactivity, Enzyme Activity
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, ZnCl2 and Glycerol.
Gene ID (Entrez) 59272
Molecular Weight (g/mol) M.W.-theroretical: 110 kDa, M.W.-observed: 122-134 kDa, under reducing conditions
Quantity 20 μg
Source Chinese Hamster Ovary cell line, CHO-derived human ACE-2 protein Human ACE-2 (Gln18-Ser740) (N-terminus) Accession # Q9BYF1.2 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Alias ACE2, ACEH, ACEHangiotensin I converting enzyme 2, ACE-related carboxypeptidase, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2, angiotensin-converting enzyme 2, Angiotensin-converting enzyme homolog, DKFZp434A014, EC 3.4.17, EC 3.4.17.23, Metalloprotease MPROT15
Conjugate Biotin
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein ACE-2
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