R&D Systems™ Recombinant Human Aggrecan G1-IGD-G2 Domains Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity, Binding Activity

The Recombinant Human Aggrecan G1-IGD-G2 Domains Protein is derived from NS0. The Recombinant Human Aggrecan G1-IGD-G2 Domains Protein has been validated for the following applications: Enzyme Activity, Binding Activity.

Specifications

• Formulation: Lyophilized from a 0.2μm filtered solution in Tris and NaCl.
• Gene ID (Entrez): 176
• Molecular Weight (g/mol): 74 kDa
• Name: Aggrecan G1-IGD-G2 Domains
• Quantity: 25 μg
• Source: Mouse myeloma cell line,NS0-derived human Aggrecan protein Val20-Gly675,with a C-terminal 10-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: For hyaluronan-binding assay, reconstitute at 100 μg/mL in PBS. For use as a metalloprotease substrate, reconstitute at 250 μg/mL in 50 mM Tris, 10 mM CaCl 2 150 mM NaCl and 0.05% Brij-35, pH 7.5.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.