R&D Systems™ Recombinant Human Agrin Protein, N-terminal

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human Agrin Protein, N-terminal is derived from CHO. The Recombinant Human Agrin Protein, N-terminal has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: O00468
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 375790
• Molecular Weight (g/mol): Observed M.W.: 138-181kDa, reducing conditions, Theoretical M.W.: 113kDa
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line,CHO-derived human Agrin protein Thr30-Arg1102,with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: agrin, agrin proteoglycan, AGRN
• Common Name: Agrin
• Gene Symbol: AGRN
• Biological Activity: Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons on a Laminin alpha 4 coated plate. Able to significantly inhibit neurite outgrowth when immobilized as a 3μL droplet containing 50 ng of protein.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Chinese Hamster Ovary cell line, CHO-derived Thr30-Arg1102, with a C-terminal 6-His tag
• Purity or Quality Grade: >95%, by SDS-PAGE with Silver Staining