R&D Systems™ Recombinant Human Angiopoietin-1 Biotinylated Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human Angiopoietin-1 Biotinylated Protein is derived from NS0. The Recombinant Human Angiopoietin-1 Biotinylated Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: Q5HYA0
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 284
• Molecular Weight (g/mol): Theoretical M.W.: 56kDa (unlabeled)kDa
• Quantity: 10 μg
• Source: Mouse myeloma cell line,NS0-derived human Angiopoietin-1 protein Ser20-Phe498,with a C-terminal 6-His tag Accession &Num; Q5HYA0
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <1.0 EU per 1μg of the protein by the LAL method.
• Gene Alias: AGP1, AGPT, Ang1, ANG-1, angiopoietin 1, angiopoietin-1, ANGPT1, KIAA0003
• Common Name: Angiopoietin-1
• Gene Symbol: ANGPT1
• Biological Activity: Measured by its ability to inhibit serum deprivation induced apoptosis in HUVEC human umbilical vein endothelial cells. Kwak, H.J. et al. (1999) FEBS Letters 448:249. The ED50 for this effect is typically 10-40ng/mL in the presence of 5μg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.
• Conjugate: Biotin
• Species: Human
• Recombinant: Recombinant
• Expression System: Mouse myeloma cell line, NS0-derived Ser20-Phe498, with a C-terminal 6-His tag Accession # Q5HYA0
• Purity or Quality Grade: >90%, by SDS-PAGE with Silver Staining