R&D Systems™ Recombinant Human B7-1/CD80 Fc Alexa Fluor™ 488 Protein

Catalog No.	Quantity
AFG10133020	20 μg

Catalog No. AFG10133020 Supplier R&D Systems™ Supplier No. AFG10133020

Description

Fc Chimera

Measured by flow cytometry for its ability to bind anti-human B7-1 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human B7-1 Fc-tag Alexa Fluor™ 488 (Catalog# AFG10133) that produces 50% of the binding response is 30.0-300 ng/mL.

Specifications

• Accession Number: P33681.1
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Gene ID (Entrez): 941
• Molecular Weight (g/mol): M.W.-observed: 66-80 kDa, under reducing conditions., M.W.-theroretical: 50.5 kDa
• Quantity: 20 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human B7-1/CD80 protein Human B7-1/CD80 (Val35-Asn242) (C-terminus) Accession # P33681.1 IEGRMD Human IgG1 (Pro100-Lys330) (N-terminus)
• Storage Requirements: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after opening. 3 months, -20°C to -70°C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Gene Alias: Activation B7-1 antigen, B7, B71, BB1, CD28LG1, CD28LGB7-1 antigen), CD80, CD80 antigen, CD80 molecule, costimulatory factor CD80, costimulatory molecule variant IgV-CD80, CTLA-4 counter-receptor B7.1, T-lymphocyte activation antigen CD80
• Conjugate: Alexa Fluor 488
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: B7-1/CD80