R&D Systems™ Recombinant Human B7-H2 Fc Chimera Protein

Catalog No.	Quantity
165B7100	100 μg

Catalog No. 165B7100 Supplier R&D Systems™ Supplier No. 165B7100

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its ability to stimulate human T cell proliferation in the presence of anti-CD3. Wang, S. et al. (2000) Blood 96:2808. The ED50 for this effect is 0.3-1.2 μg/mL in the presence of anti-CD3 immobilized at least at 20 ng/mL. Optimal dilutions should be determined by each laboratory for each application.

Specifications

• Accession Number: O75144
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 23308
• Molecular Weight (g/mol): M.W. (Observed): 80-90 kDa, reducing conditions; M.W. (theoretical): 53.4 kDa (monomer)
• Name: B7-H2 Fc Chimera
• Quantity: 100 μg
• Source: Mouse myeloma cell line, NS0-derived human B7-H2 protein Human B7-H2 (Asp19-Ser258) (N-terminus) Accession # O75144 DIEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Structural Form: Disulfide-linked homodimer
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: ICOSLG
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: B7-H2/ICOSLG