R&D Systems™ Recombinant Human B7-H3 (4Ig)/B7-H3b Fc Chimera Protein

Catalog No.	Quantity
10472B3050	50 μg

Catalog No. 10472B3050 Supplier R&D Systems™ Supplier No. 10472B3050

Description

Carrier Free Recombinant Protein

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 2-20 μg/mL.

Specifications

• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 80381
• Molecular Weight (g/mol): M.W.-observed: 100-120 kDa, under reducing conditions, M.W.-theoretical: 73 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human B7-H3 protein Human B7-H3 (4 Ig)/B7-H3b (Leu29-Thr461) (N-terminus) Accession # NP_001019907.1 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: B7H3, B7H34Ig-B7-H3, B7-H3B7 homolog 3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 200 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: B7-H3/CD276