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R&D Systems™ Recombinant Human B7-H3 Fc Chimera Biotinylated Protein

Catalog No. BT1027050
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BT1027-050 50 μg
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Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 3-12 μg/mL.

Specifications

Accession Number NP_079516
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 80381
Molecular Weight (g/mol) M.W. (Observed): 66-75 kDa, reducing conditions; M.W. (theoretical): 50 kDa (unlabeled)
Quantity 50 μg
Source Mouse myeloma cell line, NS0-derived human B7-H3 protein Human B7-H3 (Leu29-Pro245) (N-terminus) Accession # NP_079516 DIEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
Storage Requirements 12 months from date of receipt, <= -20 °C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, <= -20 °C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Conjugate Biotin
Species Human
Reconstitution Reconstitute at 200 μg/mL in PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein B7-H3/CD276
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