R&D Systems™ Recombinant Human B7-H6 Fc Chimera Biotinylated Protein

Catalog No.	Quantity
BT7144050	50 μg

Catalog No. BT7144050 Supplier R&D Systems™ Supplier No. BT7144050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its binding ability in a functional ELISA. When Recombinant Human NKp30 Fc Chimera (Catalog # 1849-NK ) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human B7-H6 Fc Chimera that produces 50% of the optimal binding response is 15-75 ng/mL. Measured by its ability to induce IFN-gamma secretion by NK-92 human natural killer lymphoma cells. Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495. The ED50 for this effect is 0.6-3 μg/mL

Specifications

• Accession Number: NP_001189368
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 374383
• Molecular Weight (g/mol): M.W. (Observed): 74-85 kDa, reducing conditions; M.W. (theoretical): 53 kDa (unlabeled)
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human B7-H6 protein Human B7-H6 (Met1-Ser262) (N-terminus) Accession # NP_001189368 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 2 weeks, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Regulatory Status: RUO
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Biotin
• Cross Reactivity: B7-H6
• Species: Human
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: B7-H6