R&D Systems™ Recombinant Human CD27 Ligand/TNFSF7 Protein

Catalog No.	Quantity
93-28C-L100	100 μg

Catalog No. 93-28C-L100 Supplier R&D Systems™ Supplier No. 9328CL100

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human CD27. The ED50 for this effect is 5-25 ng/mL in the presence of Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody (Catalog # MAB060 ).

Order Info

Ships at ambient temperature.

Specifications

• Accession Number: P32970
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Gene ID (Entrez): 970
• Molecular Weight (g/mol): M.W. (Observed): 24-32 kDa, reducing conditions; M.W. (theoretical): 23 kDa
• Quantity: 100 μg
• Source: Human embryonic kidney cell, HEK293-derived human CD27 Ligand/TNFSF7 protein HA (YPYDVPDYA) GCN4-IZ GGGSGGGSGGGS Human CD27 Ligand (Gln39-Pro193) (N-terminus) Accession # P32970 (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Regulatory Status: RUO
• Structural Form: Noncovalently-linked homotrimer
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Biological Activity: 5 to 25ng/mL
• Product Type: Recombinant protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 200 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: CD27 Ligand/TNFSF7/CD70

For Research Use Only.