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R&D Systems™ Recombinant Human CLEC4E Fc Chimera Protein

Catalog No. 8995CL050
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89-95C-L050 50μg
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Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human CLEC4E Fc Chimera Protein is derived from CHO. The Recombinant Human CLEC4E Fc Chimera Protein has been validated for the following applications: Bioactivity.

Specifications

For Use With (Application) Bioactivity
Gene ID (Entrez) 26253
Molecular Weight (g/mol) Observed M.W.: 54-64kDa, reducing conditions, Theoretical M.W.: 47kDa
Quantity 50μg
Source Chinese Hamster Ovary cell line,CHO-derived human CLEC4E protein, (N-terminus) MD Human IgG1 (Pro100-Lys330) IEGR Human CLEC4E (Arg41-Leu219) Accession &Num; Q9ULY5 (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU per 1μg of the protein by the LAL method.
Gene Alias CLECSF9, C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 9, C-type lectin domain family 4 member E, C-type lectin domain family 4, member E, Macrophage-inducible C-type lectin, MINCLE, MINCLEC-type lectin superfamily member 9
Common Name CLEC4E
Gene Symbol CLEC4E
Biological Activity Measured by its binding ability in a functional ELISA. When Trehalose 6, 6'-Dimycolate is immobilized at 1μg/mL (100μL/well), the concentration of Recombinant Human CLEC4E Fc Chimera that produces 50% of the optimal binding response is approximately 0.05-0.3μg/mL.
Conjugate Unconjugated
Species Human
Recombinant Recombinant
Expression System Chinese Hamster Ovary cell line, CHO-derived MD, N-terminus; Human IgG1 (Pro100-Lys330); IEGR; Human CLEC4E (Arg41-Leu219) Accession # Q9ULY5, C-terminus
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
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