R&D Systems™ Recombinant Human Complement Component C1rLP His Protein, CF

Description

HA-tag His-tag

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >30 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: NP_057630.1
• Formulation: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Gene ID (Entrez): 51279
• Molecular Weight (g/mol): MolecularWeight-observed: 70-76 kDa, under reducing conditions, MolecularWeight-theroretical: 52 kDa
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Complement Component C1rLP protein Cys36-Asp487 with N-terminal HA (YPYDVPDYA) and 6-His tags
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: C1RL, C1RL1, C1RL1complement C1r subcomponent-like protein, C1r-like protein, C1r-like serine protease analog protein, C1r-LPcomplement C1r-like proteinase, C1RLPEC 3.4.21.-, CLSPa, complement component 1, r subcomponent-like, EC 3.4.21, EC 3.4.21.41
• Conjugate: Unconjugated
• Protein: Complement Component C1rLP