R&D Systems™ Recombinant Human DHFR Protein

Catalog No.	Quantity
84-56D-R100	100 μg

Catalog No. 84-56D-R100 Supplier R&D Systems™ Supplier No. 8456DR100

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human DHFR Protein is derived from E. coli. The Recombinant Human DHFR Protein has been validated for the following applications: Enzyme Activity.

Specifications

• Accession Number: P00374
• For Use With (Application): Enzyme Activity
• Gene ID (Entrez): 1719
• Molecular Weight (g/mol): Observed M.W.: 23kDa, reducing conditions, Theoretical M.W.: 22kDa
• Quantity: 100 μg
• Source: Mouse myeloma cell line, NS0-derived human CD30/TNFRSF8 protein Human CD30 (Phe19-Lys379) (N-terminus) Accession # P28908.1 IEGRDMD Human IgG1 (Pro100-Lys330) 6-His tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70° C as supplied. 3 months, -20 to -70° C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU per 1μg of the protein by the LAL method.
• Gene Alias: DHFR, DHFRP1, dihydrofolate reductase, DYR, EC 1.5.1.3
• Common Name: Dihydrofolate Reductase/DHFR
• Gene Symbol: DHFR
• Biological Activity: Measured by the reduction of dihydrofolic acid (DHF). The specific activity is >5,500 pmol/min/μg, as measured under the described conditions.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: E. coli-derived Met1-Asp187, with a C-terminal 6-His tag
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.