R&D Systems™ Recombinant Human DLL1 Fc Chimera Protein

Description

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells.

ED50 for this effect is 0.1 to 0.5 μg/mL.

Specifications

• Accession Number: O00548
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 28514
• Molecular Weight (g/mol): 88 to 98 kDa, under reducing conditions
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human DLL1 protein. Human DLL1 (Ser22-Glu537) Accession # O00548IEGRMDPHuman IgG1 (Pro100-Lys330)N-terminus C-terminus
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: < 0.10 EU per 1 μg of the protein by the LAL method
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 250 μg/mL in PBS
• Buffer: Lyophilized from a 0.2 μm filtered solution in HEPES and EDTA
• Purity or Quality Grade: > 90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining