R&D Systems™ Recombinant Human EGFR Fc Chimera Alexa Fluor™ 488 Protein

Description

Recombinant Protein

Measured by flow cytometry for its ability to bind anti-human Fc gamma RIIIA/CD16a Antibody conjugated beads. The concentration of Recombinant Human Fc gamma RIIIA/CD16a Alexa Fluor 488 (Catalog# AFG4325) that produces 50% of the binding response is 15.0-150 ng/mL.

Specifications

• Accession Number: AAH17865.1
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Molecular Weight (g/mol): M.W.-observed: 40-50 kDa, under reducing conditions.; M.W.-theoretical: 22.6 kDa
• Quantity: 20 μg
• Source: Mouse myeloma cell line, NS0-derived human Fc gamma RIIIA/CD16a protein Gly17-Gln208, with a C-terminal 6-His tag
• Storage Requirements: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.
• Endotoxin Concentration: <1.0 EU per 1 μg of the protein by the LAL method.
• Conjugate: Alexa Fluor 488
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.