R&D Systems™ Recombinant Human Elongin B/Elongin C/VHL Complex

Description

Together, Elongin B (ELOB) and Elongin C (ELOC) form a heterodimer that serves as the regulatory subunit for the Elongin complex--a general transcription elongation factor that increases RNA Polymerase II transcription through template-encoded arresting sites.

Typical protein concentration for use in vitro will depend on experimental conditions.

Specifications

• Accession Number: Q15370.1 (ELOB), Q15369.1 (ELOC), P40337.2 (VHL)
• For Use With (Application): Bioactivity
• Formulation: Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.
• Molecular Weight (g/mol): M.W. (theoretical): 24 kDa (VHL), 13.1 kDa (ELOB), 12.8 kDa (ELOC)
• Purification Method: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain
• Quantity: 50 μg
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human ELOB/ELOC/VHL Complex protein Met1 - Gln118 (ELOB), Met1 - Cys112 (ELOC), Met231 - Asp443 (VHL)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method
• Conjugate: Unconjugated
• Buffer: X mg/mL (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 2 mM DTT
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: ELOB/ELOC/VHL Complex