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ACROBiosystems Recombinant Protein;Human Her2 / ErbB2 Protein;HEK293;100UG;HE2,ERBB2,CD340,HER-2,HER-2/neu,HER2,MLN19,NEU,NGL,TKR1,ERBB-2,MLN-19,TKR-1
Supplier: ACROBiosystems HE2H5225100UG
Recombinant Protein;Human Her2 / ErbB2 Protein;100UG;Human Her2, His Tag (HE2-H5225) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # AAA75493).;This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 70.2 kDa. The protein migrates as 110-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human Her2, His Tag (Cat. No. HE2-H5225) at 0.05 μg/mL (100 μL/well) can bind Trastuzumab with a linear range of 0.2-3 ng/mL (QC tested).;Herceptin (Trastuzumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human Her2, His Tag (Cat. No. HE2-H5225) with an affinity constant of 1.07 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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