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R&D Systems™ Recombinant Human Fc gamma RIIA/CD32a Avi His Protein

Catalog No. AVI1330050
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AVI1330050 50 μg
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Biotinylated Avi-tag His-tag

Measured by its binding ability in a functional ELISA. When Human IgG is immobilized at 5.00 μg/mL (100 μL/well), Biotinylated Recombinant Human Fc gamma RIIA/CD32a Avi-tag His-tag Protein binds with an ED50 of 1.00-8.00 μg/mL.

Specifications

Accession Number AAA35827.1
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 2212
Molecular Weight (g/mol) MolecularWeight-observed: 32-39 kDa, under reducing conditions.; MolecularWeight-theroretical: 24 kDa
Quantity 50 μg
Source Human embryonic kidney cell, HEK293-derived human Fc gamma RIIA/CD32a protein Human Fc gamma RIIA (Ala36-Ile218) (N-terminus) Accession # AAA35827.1 Avi-tag 6-His tag (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Alias CD32 antigen, CD32A, CD32MGC23887, CDw32fc-gamma-RIIa, Fc fragment of IgG, low affinity IIa, receptor (CD32), Fc fragment of IgG, low affinity IIa, receptor for (CD32), FCG2, Fc-gamma RII-a, Fc-gamma-RIIa, FcGR, FCGR2, FCGR2A, FCGR2A1, FcgRIIA, FcRII-a, FCRIIA, IGFR2MGC30032, IgG Fc receptor II-a, Immunoglobulin G Fc receptor II, low affinity immunoglobulin gamma Fc region receptor II-a
Conjugate Biotin
Reconstitution Reconstitute at 500 μg/mL in PBS.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein Fc gamma RIIA/CD32a
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