R&D Systems™ Recombinant Human Fc gamma RIIIA/CD16a His Avi Protein

Description

Biotinylated Isoform V176

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Human IgG is immobilized at 2 μg/mL, 100 μL/well, it binds Biotinylated Recombinant Human Fc gamma RIIIA/CD16a His-tag Avi-tag (Catalog # AVI10468) with an ED50 of 4-40 ng/mL.

Specifications

• Accession Number: AAH17865.1
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 2214
• Molecular Weight (g/mol): Observed: 48-57 kDa, under reducing conditions; Theroretical: 24 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human Fc gamma RIIIA/CD16a protein Human Fc gamma RIIIA/CD16a (Gly17-Gln208) Accession # AAH17865.1(N-terminus) 6-His tag Avi-tag N-terminus C-terminus
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Biotin
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Protein: Fc gamma RIIIA/CD16a