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R&D Systems™ Recombinant Human FGF basic/FGF2/bFGF GMP Protein, CF

Catalog No. RD10008961
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25 μg
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Catalog No. Quantity
RD10008961 25 μg
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Catalog No. RD10008961 Supplier R&D Systems™ Supplier No. BTFGFBGMP025
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Description

Recombinant Protein

Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.100-1.00 ng/mL. The specific activity of Recombinant Human FGF basic/FGF2 GMP is >8.0 x 105 IU/mg, which is calibrated against the human FGF basic/FGF2 WHO International Standard (NIBSC code: 90/712).

Specifications

Accession Number NP_001997.5
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and Sodium Sulfate with Trehalose.
Gene ID (Entrez) 2247
Molecular Weight (g/mol) Observed: 18 kDa, under reducing conditions., Observed: 17 kDa
Quantity 25 μg
Source E. coli-derived human FGF basic/FGF2/bFGF protein Ala135 - Ser288 Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines.
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20°C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, ≤ -20°C under sterile conditions after reconstitution.
Regulatory Status GMP
Endotoxin Concentration <0.01 EU per 1 μg of the protein by the LAL method.
Gene Alias Basic fibroblast growth factor, basic fibroblast growth factor bFGF, bFGF, FGF2, FGF-2, FGFBprostatropin, fibroblast growth factor 2 (basic), HBGF-2, heparin-binding growth factor 2, Prostatropin
Conjugate Unconjugated
Recombinant Recombinant
Reconstitution Reconstitute 25 μg size at 250 μg/mL and all other sizes at 500 μg/mL in sterile water.
Purity or Quality Grade >97%, by SDS-PAGE with quantitative densitometry by Coomassie™ Blue Staining. The primary molecular weight by mass spectrometry is 17112 Da ± 10 Da
Protein FGF basic/FGF2/bFGF
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