R&D Systems™ Recombinant Human FGF basic/FGF2 Protein, CF

Description

Recombinant Protein

Measured by its ability to inhibit human FGF-basic-dependent proliferation of NR6R-3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266; Thomas, K. et al. (1987) Methods Enzymol. 147:120; Watson, J.B. et al. (1994) J. Clin. Invest. 94:261. The ED50 for this effect is 0.100-1.00 ng/mL.

Specifications

• Accession Number: NP_001997.5
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in HEPES and Sodium Sulfate with Trehalose.
• Gene ID (Entrez): 2247
• Molecular Weight (g/mol): MolecularWeight-observed: 18 kDa, under reducing conditions., MolecularWeight-theroretical: 16 kDa
• Quantity: 500 μg
• Source: E. coli-derived human FGF basic/FGF2/bFGF protein Ala135-Ser288
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.01 EU per 1 μg of the protein by the LAL method.
• Gene Alias: Basic fibroblast growth factor, basic fibroblast growth factor bFGF, bFGF, FGF2, FGF-2, FGFBprostatropin, fibroblast growth factor 2 (basic), HBGF-2, heparin-binding growth factor 2, Prostatropin
• Conjugate: Unconjugated
• Reconstitution: Reconstitute the 20 μg size at 100 μg/mL in sterile deionized water. Reconstitute all other sizes at 500 μg/mL in sterile deionized water.
• Purity or Quality Grade: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: FGF basic/FGF2/bFGF