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R&D Systems™ Recombinant Human FGFR1 alpha (IIIc) Fc Avi-tag Protein

Catalog No. AVI11031050
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AVI11031050 50 μg
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Biotinylated

Measured by its binding ability in a functional ELISA. In a Human FGF acidic/FGF1 antibody (Catalog # AF232 ) coated plate, in the presence of 50.0 ng/mL of Recombinant Human FGF acidic/FGF1, Biotinylated Recombinant Human FGFR1 alpha (IIIc) Fc Chimera Avi-tag Protein binds with an ED50 of 0.100-0.600 μg/mL.

Specifications

Accession Number NP_075598.2
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 2260
Molecular Weight (g/mol) MolecularWeight-observed: 100-120 kDa, under reducing conditions., MolecularWeight-theroretical: 68 kDa
Quantity 50 μg
Source Chinese Hamster Ovary cell line, CHO-derived human FGFR1 alpha protein Human FGF R1 alpha (IIIc) (Arg22-Glu376) (N-terminus) Accession # NP_075598.2 IEGRDMD Human IgG 1 (Pro100-Lys330) Avi-tag (C-terminus)
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Conjugate Biotin
Reconstitution Reconstitute at 500 μg/mL in PBS.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein FGFR1 alpha
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