R&D Systems™ Recombinant Human Fibronectin, ACFP Protein

Catalog No.	Quantity
ACFP4305B01	1 mg

Catalog No. ACFP4305B01 Supplier R&D Systems™ Supplier No. ACFP4305B01M

Description

Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture.

• Recommended concentration for this effect is typically 1 to 5 μg/cm2.
• Fibronectin can also be added to the media to support cell spreading at a concentration of 0.5 to 50 μg/mL.
• Optimal concentrations will need to be determined for individual user applications

Specifications

• Accession Number: CAE45932
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 2335
• Molecular Weight (g/mol): 180 to 250 kDa, reducing conditions
• Quantity: 1 mg
• Source: Spodoptera frugiperda, Sf 9 (baculovirus)-derived human Fibronectin protein Met1-Pro1908, with a C-terminal 6-His tag Produced in an animal component free process (ACFP)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: < 0.10 EU per 1 μg of the protein by the LAL method
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 100 μg/mL in sterile water
• Buffer: Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl and Tween™
• Purity or Quality Grade: > 95%, by SDS-PAGE under reducing conditions and visualized by silver Stain