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R&D Systems™ Recombinant Human GDF-15 (CHO-expressed) Protein

Catalog No. 957GD025CF
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Formulation:
Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA.
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Catalog No. Formulation
957GD025CF Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA.
957GD025 Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
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Catalog No. 957GD025CF Supplier R&D Systems™ Supplier No. 957GD025/CF
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Description

Extensive quality control produces lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to activate SRE-SEAP reporter in HEK293 human embryonic kidney cells transfected with human c-Ret and human GFRAL. The ED50 for this effect is 0.4-4 ng/mL.

The Recombinant Human GDF-15 Protein is derived from CHO.

Specifications

Formulation Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA.
Gene ID (Entrez) 9518
Molecular Weight (g/mol) Predicted MW: 13kDa (monomer) Observed MW: 14kDa, reducing conditions
Name GDF-15
Quantity 25 μg
Source Chinese Hamster Ovary cell line,CHO-derived human GDF-15 protein Ala197-Ile308,with an N-terminal 6-His tag
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
Structural Form Disulfide-linked homodimer
Conjugate Unconjugated
Recombinant Recombinant
Reconstitution Reconstitute at 100-300μg/mL in sterile 4mM HCI.
Purity or Quality Grade 97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
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