R&D Systems™ Recombinant Human Glucokinase/GCK Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to transfer phosphate from ATP to glucose. The specific activity is >265 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: P35557
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl, Brij-35 and DTT.
• Gene ID (Entrez): 2645
• Molecular Weight (g/mol): M.W. (Observed): 60-65 kDa, reducing conditions; M.W. (theoretical): 63 kDa
• Name: Glucokinase/GCK
• Quantity: 20 μg
• Source: E. coli-derived human Glucokinase/GCK protein Met 6-His tag Human Cystatin A (Met1-Phe98) (N-terminus) Accession # P01040 GS Human Glucokinase/GCK (Val16-Gln465) (N-terminus) Accession # P35557 (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: GCK
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain (at 5 μg / lane.)
• Protein: Glucokinase/GCK