R&D Systems™ Recombinant Human HGF (NS0 Expressed) Protein

Catalog No.	Quantity
11-352-HG010	10 μg

Catalog No. 11-352-HG010 Supplier R&D Systems™ Supplier No. 11352HG010

Description

Wild type

Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED50 for this effect is equal to or less than 4.00 ng/mL.

Specifications

• Accession Number: P14210.2
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2μm filtered solution in PBS and NaCl with Trehalose.
• Gene ID (Entrez): 3082
• Molecular Weight (g/mol): M.W. (Observed): 30-40 kDa ( beta chain) & 54-66 kDa ( alpha chain), under reducing conditions., M.W. (Theoretical): 54 kDa ( alpha chain) & 26 kDa ( beta chain)
• Quantity: 10 μg
• Source: Mouse myeloma cell line, NS0-derived human HGF protein Gln32-Arg494 alpha chain & Val495-Ser728 beta chain
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.50 EU per 1μg of the protein by the LAL method.
• Gene Alias: deafness, autosomal recessive 39, DFNB39, EC 3.4.21, EC 3.4.21.7, fibroblast-derived tumor cytotoxic factor, F-TCF, hepatocyte growth factor (hepapoietin A; scatter factor), Hepatopoeitin-A, Hepatopoietin A, HGFB, HPTA, HPTAhepatocyte growth factor, lung fibroblast-derived mitogen, Scatter factor, SF, SFhepatopoeitin-A
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute 10μg size at 100μg/mL in PBS. Reconstitute all the other sizes at 200μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: HGF