R&D Systems™ Recombinant Human His6-SENP1 Catalytic Domain Protein

Catalog No.	Quantity
E700050	50 μg

Catalog No. E700050 Supplier R&D Systems™ Supplier No. E700050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

Recombinant Human His6-SENP1 Catalytic Domain is a SUMO-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-SENP1 Catalytic Domain concentration of 50-500 nM. A 15 minute pre-incubation with 10 mM DTT is recommended to achieve maximum activity.

Specifications

• Accession Number: Q9P0U3
• For Use With (Application): Enzyme Activity
• Formulation: X mg/mL (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% Glycerol (v/v), 5 mM DTT
• Gene ID (Entrez): 29843
• Molecular Weight (g/mol): M.W. (theoretical): 30 kDa
• Name: His6 SENP1 Catalytic Domain
• Quantity: 50 μg
• Source: E. coli-derived human SENP1 protein Contains an N-terminal Gly-Ser-Ser and 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Gene Symbol: SENP1
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Buffer: X mg/mL (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% Glycerol (v/v), 5 mM DTT
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: SENP1