R&D Systems™ Recombinant Human IL-15 Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 0.3-2.6 ng/mL. The specific activity of Recombinant Human IL-15 is approximately 4.5 x 105 U/μg, which is calibrated against recombinant human IL-15 WHO International Standard (NIBSC code: 95/554). Specific activity is for reference purposes only and is not routinely tested.

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Specifications

• Accession Number: P40933.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2μm filtered solution in PBS with BSA as a carrier protein. Reconstitute at 100μg/mL in sterile PBS.
• Gene ID (Entrez): 3600
• Molecular Weight (g/mol): 10 kDa, under reducing conditions.
• Quantity: 5 μg
• Source: E. coli-derived human IL-15 protein Asn49-Ser162
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: IL15, IL-15MGC9721, interleukin 15, interleukin-15
• Common Name: IL-15
• Gene Symbol: IL15
• Biological Activity: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 0.3-2.6ng/mL. The specific activity of Recombinant Human IL-15 is approximately 4.5 x 105 U/μg, which is calibrated against recombinant human IL-15 WHO International Standard (NIBSC code: 95/554).
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: E. coli-derived Asn49-Ser162
• Purity or Quality Grade: >97%, by SDS-PAGE with Silver Staining