R&D Systems™ Recombinant Human IL-18 R beta/IL-1 R7 Fc Avi Protein

Catalog No.	Quantity
AVI118050	50 μg

Catalog No. AVI118050 Supplier R&D Systems™ Supplier No. AVI118050

Description

Biotinylated

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera (Catalog # 816-LR ) is coated at 1.00 μg/mL (100 μL/well), in the presence of 50.0 ng/mL of Recombinant Human IL-18 (Catalog # 9124-IL ), Biotinylated Recombinant Human IL-18 R beta/IL-1 R7 Fc Chimera Avi-tag binds with an ED50 of 10.0-100 ng/mL. The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.

Specifications

• Accession Number: O95256.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 8807
• Molecular Weight (g/mol): M.W.-observed: 87-97 kDa, under reducing conditions., M.W.-theroretical: 65 kDa (monomer)
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human IL-18 R beta/IL-1 R7 protein Human IL-18 R beta/IL-1 R7(Phe20-Arg356)(N-terminus) Accession # O95256.1 DIEGRMD Human IgG1(Pro100-Lys330) Avi-tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: Accessory protein-like, AcPL, CD218 antigen-like family member B, CD218b, CD218b antigen, CDw218b, IL-1 R7, IL-18 receptor accessory protein, IL-18 receptor beta, IL18R beta, IL-18 RAcP, IL18RAP, IL-18 Rb, IL18RB, IL-18 Rbeta, IL-18 R-beta, IL-1 R accessory protein-like, IL1R7, IL-1R7, IL-1 R-7, IL-1 RAcPL, interleukin 18 receptor accessory protein, Interleukin-1 receptor 7, interleukin-18 receptor accessory protein, Interleukin-18 receptor accessory protein-like, Interleukin-18 receptor beta, MGC120589, MGC120590
• Conjugate: Biotin
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: IL-18 R beta/IL-1 R7/ACPL