R&D Systems™ Recombinant Human IL-2, Animal-Free Protein

Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells

Catalog Number	Mfr. No.	Quantity
AFL202010	R&D Systems™ AFL202010	10 μg
AFL202050	R&D Systems™ AFL202050	50 μg

Description

Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.05-0.25 ng/mL. The specific activity of Recombinant Human IL-2 is approximately 2.1 x 104 IU/μg, which is calibrated against recombinant human IL-2 WHO International Standard (NIBSC code: 86/500).

Specifications

• Accession Number: P60568.1
• Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
• Molecular Weight (g/mol): M.W. (theoretical): 15 kDa
• Source: E. coli-derived human IL-2 protein Ala21-Thr153, with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory.
• Endotoxin Concentration: <0.01 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: IL2
• Species: Human
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with Trehalose.
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 3558
• Name: aldesleukin, IL2, IL-2lymphokine, interleukin 2, interleukin-2, involved in regulation of T-cell clonal expansion, T cell growth factor, T-cell growth factor, TCGF
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, <= -20 °C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, <= -20 °C under sterile conditions after reconstitution.
• Gene Alias: aldesleukin, IL2, IL-2 lymphokine, interleukin 2, interleukin-2, involved in regulation of T-cell clonal expansion, T cell growth factor, T-cell growth factor, TCGF
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile 100 mM Acetic Acid. Alternatively, reconstitute at 0.1 mg/mL in sterile deionized water and use within 24 hours, store (at 2°C to 8°C.)
• Endotoxin Level: <0.01 EU per 1 μg of the protein by the LAL method.
• Protein: IL-2