R&D Systems™ Recombinant Human IL-3 GMP Protein, CF

Catalog No.	Quantity
BT003GMP050	50 μg

Catalog No. BT003GMP050 Supplier R&D Systems™ Supplier No. BT003GMP050

Description

Recombinant Protein

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.0150-0.150 ng/mL. The specific activity of GMP Recombinant Human IL-3 is >1.7 x 106 IU/mg, which is calibrated against the human IL-3 WHO International Standard (NIBSC code: 91/510).

Specifications

• Accession Number: AAC08706.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 3562
• Molecular Weight (g/mol): MolecularWeight-observed: 14 kDa, under reducing conditions., MolecularWeight-theroretical: 15 kDa
• Quantity: 50 μg
• Source: E. coli-derived human IL-3 protein Ala20 - Phe152, with and without an N-terminal Met Produced using non-animal reagents in an animal-free laboratory. Manufactured and tested under cGMP guidelines.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: Hematopoietic growth factor, IL3, IL-3MGC79398, interleukin 3 (colony-stimulating factor, multiple), interleukin-3, Mast cell growth factor, mast-cell growth factor, MCGF, MCGFMGC79399, MULTI-CSF, multilineage-colony-stimulating factor, Multipotential colony-stimulating factor, P-cell stimulating factor, P-cell-stimulating factor
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in sterile water.
• Purity or Quality Grade: >97%, by SDS-PAGE with quantitative densitometry by Coomassie™ Blue Staining. The molecular weight by mass spectrometry is 15201 Da ± 50 Da, and a second 15070 Da ± 50 Da product may be present.
• Protein: IL-3