R&D Systems™ Recombinant Human IL-6 (HEK293-expressed) Biotin Protein

Catalog No.	Quantity
BT7270B050	50 μg

Catalog No. BT7270B050 Supplier R&D Systems™ Supplier No. BT7270B050

Description

Biotinylated

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-6R alpha His-tag (Catalog # 10537-SR ) is immobilized at 0.25 μg/mL (100 μL/well), Biotinylated Recombinant Human IL-6 (Catalog # BT7270B) binds with an ED50 of 2.00-16.0 ng/mL.

Specifications

• Accession Number: P05231.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 3569
• Molecular Weight (g/mol): M.W.-observed: 21-30 kDa, under reducing conditions., M.W.-theroretical: 21 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human IL-6 protein Val30-Met212
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor, HSF, hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6, Interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2 A
• Conjugate: Biotin
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: IL-6