R&D Systems™ Recombinant Human IL-6/IL-6R alpha Protein Chimera

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

The Recombinant Human IL-6/IL-6R alpha Protein Chimera is derived from HEK293. The Recombinant Human IL-6/IL-6R alpha Protein Chimera has been validated for the following applications: Bioactivity.

Specifications

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 3570
• Molecular Weight (g/mol): Observed M.W.: 78 - 93kDa, reducing conditions, Theoretical M.W.: 59kDa
• Name: IL-6R alpha
• Quantity: 25 μg
• Source: Human embryonic kidney cell,HEK293-derived human IL-6/IL-6R alpha Complex protein, (N-terminus) Human IL-6R alpha (Leu20-Asp358) Accession &Num; P08887 GGGSGGGSGGGS Human IL-6 Val30-Met212 Accession &Num; P05231 (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Gene Alias: BSF-2, CD126, gp80, IL6, IL-6 R alpha, IL6Q, IL6R, IL6R alpha, IL-6R-1, IL6RA, IL-6RA, IL6RQ, interleukin 6 receptor, MGI-2A
• Common Name: IL-6 R alpha
• Gene Symbol: IL6
• Biological Activity: Measured by its ability to inhibit M1 mouse myeloid leukemia cell proliferation. The ED50 for this effect is typically 2.5-12.5ng/mL.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Expression System: Human embryonic kidney cell, HEK293-derived Human IL-6 R alpha (Leu20-Asp358) Accession # P08887, N-terminus; GGGSGGGSGGGS; Human IL-6 Val30-Met212 Accession # P05231, C-terminus
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining
• Protein: IL-6/IL-6 R alpha Complex