R&D Systems™ Recombinant Human IL-7 (HEK-293-expressed) Protein

Description

Recombinant Protein

Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The ED50 for this effect is 0.100-0.500 ng/mL. The specific activity of Recombinant Human IL-7 is >1.00 x 108 units/mg, which is calibrated against the human IL-7 reference standard (NIBSC code: 90/530). Specific activity is for reference purposes only and is not routinely tested.

Specifications

• Accession Number: P13232.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 3574
• Molecular Weight (g/mol): MolecularWeight-observed: 18-28 kDa, under reducing conditions.; MolecularWeight-theroretical: 17 kDa
• Quantity: 10 μg
• Source: Human embryonic kidney cell, HEK293-derived human IL-7 protein Asp26 - His177
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: IL7, IL-7 interleukin-7, interleukin 7, Lymphopoietin-1, PBGF
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 - 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: IL-7