R&D Systems™ Recombinant Human Integrin a5ss1 Protein, CF

Catalog No.	Quantity
32-30A-5B050	50 μg

Catalog No. 32-30A-5B050 Supplier R&D Systems™ Supplier No. 3230A5B050

Description

Recombinant Protein

Measured by its binding ability in a functional ELISA. Recombinant Human Integrin alpha 5 beta 1 (Catalog # 3230-A5B) binds Human Fibronectin (Catalog # 1918-FN ) with an ED50 of 1.00-12.0 ng/mL.

Specifications

• Accession Number: P08648 & P05556
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 3678
• Molecular Weight (g/mol): MolecularWeight-observed: 110-140 kDa, under reducing conditions., MolecularWeight-theroretical: 108 kDa ( alpha 5 subunit), 82.3 kDa ( beta 1 subunit)
• Quantity: 50 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 5 beta 1 protein Human Integrin alpha 5 (Phe42-Tyr995) (N-terminus) Accession # P08648 Acidic Tail Human Integrin beta 1 (Gln21-Asp728) (N-terminus) Accession # P05556 Basic Tail (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.20 EU per 1 μg of the protein by the LAL method.
• Gene Alias: CD49 antigen-like family member E, CD49e, Fibronectin receptor subunit alpha, fibronectin receptor, alpha polypeptide, fibronectin receptor, alpha subunit, FNRA, integrin alpha-5, Integrin alpha-F, integrin, alpha 5 (fibronectin receptor, alpha polypeptide), ITGA5/SLC9B1 fusion, very late activation protein 5, alpha subunit, VLA-5, VLA5A
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Integrin alpha 5 beta 1