R&DSystems™ Recombinant Human Integrin alpha 5 beta 1 Fc Protein, CF

Description

Fc Chimera

Measured by its binding ability in a functional ELISA. Recombinant Human Integrin alpha 5 beta 1 Fc Chimera (Catalog # 11538-A5) binds Human Fibronectin (Catalog # 1918-FN ) with an ED50 of 0.600-9.00 ng/mL.

Specifications

• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 3678
• Molecular Weight (g/mol): M.W.-observed: 225-370 kDa, under non-reducing conditions, M.W.-theroretical: 131 kDa (Integrin alpha 4) & 105 kDa (Integrin beta 1)
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human Integrin alpha 5 beta 1 protein Human ITGA5 (Phe42-Tyr995) (N-terminus) Accession # P08648.2 IEGR Human IgG1 (Pro99-Lys330) (with modifications) Human ITGB1 (Gln21-Asp728) (N-terminus) Accession # P05556.2 IEGR Human IgG1 (Pro99-Lys330) (with modifications) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: CD49 antigen-like family member E, CD49e, Fibronectin receptor subunit alpha, fibronectin receptor, alpha polypeptide, fibronectin receptor, alpha subunit, FNRA, integrin alpha-5, Integrin alpha-F, integrin, alpha 5 (fibronectin receptor, alpha polypeptide), ITGA5/SLC9B1 fusion, very late activation protein 5, alpha subunit, VLA-5, VLA5A
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Integrin alpha 5 beta 1