R&D Systems™ Recombinant Human ISG15 AMC Protein

Catalog No.
UL553050	50ug; Carrier Free

Catalog No. UL553050 Supplier R&D Systems™ Supplier No. UL553050

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity

This fluorogenic substrate for ISG15 hydrolases is based on the carboxy-terminus derivatization of ISG15 with 7-amido-4-methylcoumarin (AMC). Release of AMC fluorescence can be monitored with an excitation wavelength of 380 nm and an emission wavelength of 460 nm. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human ISG15 AMC concentration of 0.1-1 µM.

Specifications

• Accession Number: P05161.5
• Formulation: Supplied as a 0.2 μm filtered solution in HEPES, NaCl, Glycerol and DTT.
• Gene ID (Entrez): 9636
• Molecular Weight (g/mol): M.W. Theroretical: 18 kDa
• Name: ISG15/UCRP AMC
• Quantity: 50μg
• Source: E. coli-derived human ISG15/UCRP protein Met1 - Gly156 (C78S, G157C) with a C-terminal 6-His tag; C-terminal AMC derivatized protein
• Storage Requirements: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Conjugate: 7-Amino-4-Methyl Coumarin
• Recombinant: Recombinant