R&D Systems™ Recombinant Human LAG-3 Fc Chimera Biotinylated Protein

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human LSECtin/CLEC4G (Catalog # 2947-CL ) is coated at 1 μg/mL, 100 uL/well, biotinylated recombinant LAG-3 Fc Chimera (Catalog# BT2319) binds with an ED50 of 0.2-2 μg/mL.

Specifications

• Accession Number: P18627
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 3902
• Molecular Weight (g/mol): M.W. (Observed): 74-91 kDa, reducing conditions; M.W. (theoretical): 72.7 kDa
• Quantity: 50 μg
• Source: Mouse myeloma cell line, NS0-derived human LAG-3 protein Human LAG-3 (Leu23-Leu450) (N-terminus) Accession # P18627 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Regulatory Status: RUO
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Specific Reactivity: LAG-3
• Conjugate: Biotin
• Species: Human
• Recombinant: Recombinant
• Reconstitution: Reconstitute at 200 μg/mL in PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: LAG-3