R&D Systems™ Recombinant Human Lysosomal alpha-Glucosidase/GAA

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Measured by its ability to release glucose from starch. The specific activity is >7,500 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: P10253
• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
• Gene ID (Entrez): 2548
• Molecular Weight (g/mol): M.W. (Observed): 95-105 kDa, reducing conditions; M.W. (theoretical): 99 kDa
• Name: Lysosomal alpha-Glucosidase
• Quality Control Testing: SDS-PAGE: 95-105kDa, reducing conditions
• Quantity: 25 μg
• Source: Human embryonic kidney cell, HEK293-derived human Lysosomal alpha-Glucosidase protein Ala70-Cys952, with an N-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 6 months, -20°C to -70°C under sterile conditions after opening.
• Regulatory Status: RUO
• Termination: His
• Without Additives: Carrier Protein
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: GAA
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain (at 5 μg / lane.)
• Protein: LYAG/GAA