R&D Systems™ Recombinant Human M-CSF, Animal-Free Protein

Description

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells

• ED50 for this effect is 0.5 to 1.5 ng/mL.
• The specific activity of Recombinant Human M-CSF is approximately 1.68 x 105 IU/μg, which is calibrated against recombinant human M-CSF WHO International Standard (NIBSC code: 89/512).

Specifications

• Accession Number: NP_757350.2
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 1435
• Molecular Weight (g/mol): 18.5 kDa (monomer)
• Name: colony stimulating factor 1 (macrophage), CSF1, CSF-1, Lanimostim, macrophage colony stimulating factor, macrophage colony-stimulating factor 1, MCSF, M-CSF, MCSFlanimostim, MGC31930
• Quantity: 5 μg
• Source: E. coli-derived human M-CSF protein Glu33-Ser190, with an N-terminal Met Produced using non-animal reagents in an animal-free laboratory.
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Gene Symbol: CSF1
• Conjugate: Unconjugated
• Species: Human
• Reconstitution: Reconstitute at 0.2 mg/mL in sterile PBS.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Endotoxin Level: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.