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R&D Systems™ Recombinant Human M-CSF Biotinylated Protein

Catalog No. BT216025
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25 μg
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BT216025 25 μg
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Biotinylated

Measured by its binding ability in a functional ELISA. When Recombinant Human M-CSF R/CD115 Fc Chimera Protein (Catalog # 329-MR ) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of &Biotinylated Recombinant Human M-CSF (Catalog # BT216) that produces 50% of the optimal binding response is 1.50-15.0 ng/mL.

Specifications

Accession Number NP_757350.2
For Use With (Application) Bioactivity
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Gene ID (Entrez) 1435
Molecular Weight (g/mol) MolecularWeight-observed: 15-22 kDa, under reducing conditions.; MolecularWeight-theroretical: 18.5 kDa
Quantity 25 μg
Source E. coli-derived human M-CSF protein Glu33-Ser190, with an N-terminal Met
Storage Requirements Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
Endotoxin Concentration <0.10 EU / 1 μg of the protein by the LAL method.
Gene Alias colony stimulating factor 1 (macrophage), CSF1, CSF-1, Lanimostim, macrophage colony stimulating factor, macrophage colony-stimulating factor 1, MCSF, M-CSF, MCSFlanimostim, MGC31930
Conjugate Biotin
Reconstitution Reconstitute at 250 μg/mL in PBS.
Purity or Quality Grade >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Protein M-CSF
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