R&D Systems™ Recombinant Human MMP-9 His-tag Avi-tag Protein, CF

Catalog No.
AVI911020	20 μg

Catalog No. AVI911020 Supplier R&D Systems™ Supplier No. AVI911020

Description

Recombinant Protein

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001 ). The specific activity is >1500 pmol/min/μg, as measured under the described conditions. Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human MMP-9 His-tag Avi-tag (Catalog # AVI911) binds Human/Primate MMP-9 Antibody (Catalog # MAB936R ) with an ED50 of 1.25-15.0 ng/mL.

Specifications

• For Use With (Application): Enzyme Activity
• Formulation: Supplied as a 0.2 μm filtered solution in Tris, CaCl2, NaCl and Brij-35.
• Gene ID (Entrez): 4318
• Molecular Weight (g/mol): M.W.-observed: 90-100 kDa, under reducing conditions, M.W.-theroretical: 79.0 kDa
• Quantity: 20 μg
• Source: Chinese Hamster Ovary cell line, CHO-derived human MMP-9 protein Human MMP-9 (Ala20-Asp707) Accession # (N-terminus) P14780.1 6-His tag GG Avi-tag (C-terminus)
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: 92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, EC 3.4.24, EC 3.4.24.35, Gelatinase B, GELB, macrophage gelatinase, MANDP2, matrix metallopeptidase 9, matrix metalloproteinase 9, matrix metalloproteinase-9, MMP9, MMP-9, type V collagenase
• Conjugate: Biotin
• Protein: MMP-9