R&D Systems™ Recombinant Human Osteopontin/OPN-a His-tag Protein, CF

Description

Recombinant Protein

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. The ED50 for this effect is 0.100-1.20 μg/mL.

Specifications

• Accession Number: P10451.1
• For Use With (Application): Bioactivity
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Gene ID (Entrez): 6696
• Molecular Weight (g/mol): MolecularWeight-observed: 60-66 kDa, under reducing conditions., MolecularWeight-theroretical: 35 kDa
• Quantity: 50 μg
• Source: Human embryonic kidney cell, HEK293-derived human Osteopontin/OPN protein Ile17-Asn314 with a C-terminal 6-His tag
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU per 1 μg of the protein by the LAL method.
• Gene Alias: BNSP, Bone sialoprotein 1, Eta-1, MGC110940, Nephropontin, OPN, osteopontin, secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1), secreted phosphoprotein-1 (osteopontin, bone sialoprotein), Spp1, SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, uropontin
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Purity or Quality Grade: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
• Protein: Osteopontin/OPN