R&D Systems™ Recombinant Human Parkin pS65 Protein

Catalog No.	Quantity
E3166025	25 μg

Catalog No. E3166025 Supplier R&D Systems™ Supplier No. E3166025

Description

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Reaction conditions will need to be optimized for each specific application. Unlike unphosphorylated Parkin (E3-160), Parkin pS65 has substantial ligase activity in the absence of added activators as determined by its ability to autoubiquitinate, and ubiquitinate a Miro/Rhot1 substrate (SP-490).

Specifications

• Accession Number: O60260
• For Use With (Application): Bioactivity
• Formulation: X mg/mL (X μM) in 25 mM Tris pH 8.5, 200 mM NaCl, 0.03% Brij35, 10% (v/v) Glycerol, 5 mM TCEP
• Gene ID (Entrez): 5071
• Molecular Weight (g/mol): M.W. (theoretical): 52 kDa
• Quantity: 25 μg
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Parkin protein
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
• Regulatory Status: RUO
• Specific Reactivity: Parkin
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Buffer: X mg/mL (X μm) in 25 mM Tris pH 8.5, 200 mM NaCl, 0.03% Brij35, 10% (v/v) Glycerol, 5 mM TCEP
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain.
• Protein: Parkin